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Signaling of lysophosphatidic acid-evoked chloride current: calcium release from inositol trisphosphate-sensitive
K Kakizawa1, H Nomura, A Yoshida
1Department of Molecular Pharmacology and Neuroscience, Nagasaki University, School of Pharmaceutical Sciences, 1-14 Bunkyo-machi, Nagasaki 852-8521, Japan.
Abstract:
In Xenopus oocytes, lysophosphatidic acid (LPA) evoked inward currents at the holding potential of -60 mV, which were quickly desensitized upon repeated challenges of the compound at 10 nM or 1 microM. This desensitization was prevented by pretreatment with protein kinase A inhibitor or recovered by its post-treatment, but not by the pretreatment with an inhibitor of protein kinase C or calmodulin kinase II. From pharmacological studies, the LPA-evoked currents were found to be mediated by phospholipase C, calcium-mobilization from thapsigargin-sensitive Ca2+ stores, araguspongine E-sensitive inositol trisphosphate receptor, and calcium-dependent chloride channels.