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Related Experiment Videos

Aromatase expression in the human breast

A Brodie1, B Long, Q Lu

  • 1Department of Pharmacology and Experimental Therapeutics, Greenebaum Cancer Center, School of Medicine, University of Maryland, Baltimore 21201, USA.

Breast Cancer Research and Treatment
|October 31, 1998
PubMed
Summary
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Breast cancer cells can produce their own estrogen using aromatase (an enzyme). This local estrogen production is linked to increased tumor growth and proliferation, suggesting a significant role in breast cancer development.

Area of Science:

  • Endocrinology
  • Oncology
  • Molecular Biology

Background:

  • Postmenopausal women have low plasma estradiol but high breast tissue estrogen.
  • Breast cells may locally synthesize estrogens, with tumor aromatase potentially driving tumor growth.

Purpose of the Study:

  • Investigate aromatase expression in normal breast tissue and breast cancers.
  • Determine the role of local estrogen synthesis in breast tumor proliferation.

Main Methods:

  • Developed an immunocytochemical method using monoclonal antibodies to detect aromatase.
  • Used in situ hybridization to detect aromatase mRNA (P450 arom) for specificity verification.
  • Assessed aromatase activity, proliferating cell nuclear antigen (PCNA) expression, and thymidine incorporation in tumors.

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Main Results:

  • Aromatase enzyme and mRNA were found in epithelial and stromal cells of normal breast tissue and breast cancers.
  • Aromatase activity correlated with PCNA expression in tumors.
  • Tumors with high aromatase activity showed increased thymidine incorporation in response to testosterone.

Conclusions:

  • Local estrogen production by breast tumor aromatase is significant.
  • Locally produced estrogen enhances tumor cell proliferation.
  • Aromatase expression and activity are important factors in breast cancer growth.