Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A novel screening method for isolating exopolysaccharide-deficient mutants

M Liu1, J E González, L B Willis

  • 1Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.

Applied and Environmental Microbiology
|October 31, 1998
PubMed
Summary

Researchers developed a new staining method using Sudan Black B to identify Rhizobium meliloti mutants lacking exopolysaccharides. This technique efficiently isolated mutants deficient in succinoglycan or EPS II production.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Desmoid tumor: Clinical analysis and literature review.

Revista de gastroenterologia de Mexico (English)·2025
Same author

The antihypertensive and diuretic effect of crude root extract and saponins from Solanum sisymbriifolium Lam., in L-NAME-induced hypertension in rats.

Journal of ethnopharmacology·2022
Same author

Erratum: Azimuthal Anisotropy at the Relativistic Heavy Ion Collider: The First and Fourth Harmonics [Phys. Rev. Lett. 92, 062301 (2004)].

Physical review letters·2021
Same author

Uncertainty calculation methods in dose assessment for dicentric chromosome assay.

International journal of radiation biology·2020
Same author

A note on Poisson goodness-of-fit tests for ionizing radiation induced chromosomal aberration samples.

International journal of radiation biology·2018
Same author

The Latin American Biological Dosimetry Network (LBDNet).

Radiation protection dosimetry·2016

Area of Science:

  • Microbiology
  • Bacteriology
  • Molecular Biology

Background:

  • Exopolysaccharides (EPS) are crucial for bacterial survival and interaction with hosts.
  • Identifying mutants defective in EPS production is essential for understanding their function.
  • Rhizobium (Sinorhizobium) meliloti produces multiple EPS, including succinoglycan and EPS II.

Purpose of the Study:

  • To describe a novel screening method for isolating exopolysaccharide-deficient mutants.
  • To apply this method to Rhizobium (Sinorhizobium) meliloti.
  • To assess the potential utility of this method for other bacterial species.

Main Methods:

  • Differential staining using the lipophilic dye Sudan Black B.
  • Comparison of wild-type Rhizobium (Sinorhizobium) meliloti with exopolysaccharide-deficient mutants.

Related Experiment Videos

  • Isolation and characterization of mutants lacking specific EPS (succinoglycan or EPS II).

    • Main Results:

    • Successfully identified and isolated mutants with defects in succinoglycan production.
    • Successfully identified and isolated mutants with defects in EPS II (galactoglucan) production.
    • The Sudan Black B staining method proved effective for differentiating EPS-producing and non-producing strains.

    Conclusions:

    • A simple and effective screening method for isolating exopolysaccharide-deficient mutants of Rhizobium (Sinorhizobium) meliloti has been established.
    • This differential staining technique offers a valuable tool for bacterial genetics and physiology research.
    • The method shows promise for broader application in isolating EPS-defective mutants from diverse bacterial genera.