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Rat gastric procolipase: sequence, expression, and secretion during high-fat feeding
M S Winzell1, M E Lowe, C Erlanson-Albertsson
1Section for Molecular Signaling, Department of Cell and Molecular Biology, University of Lund, Lund, Sweden. Maria.Sorhede@medkem.lu.se
Gastroenterology
|October 31, 1998
Summary
Rat gastric procolipase sequence was determined and found identical to pancreatic procolipase. High-fat diets reduced its expression, and gastric procolipase activation releases colipase and enterostatin.
Area of Science:
- Gastroenterology
- Molecular Biology
- Biochemistry
Background:
- Procolipase, a pancreatic lipase cofactor, was identified in rat gastric tissue.
- Understanding gastric procolipase function and regulation is crucial for digestive physiology.
Purpose of the Study:
- Determine the sequence of rat gastric procolipase.
- Evaluate gastric procolipase expression and secretion under high-fat feeding.
- Investigate the activation of gastric procolipase into colipase and enterostatin.
Main Methods:
- Cloning of gastric procolipase cDNA from a rat library.
- High-fat diet induction in rats with subsequent tissue and gastric juice collection.
- Analysis of gastric juice for procolipase, colipase, and enterostatin using HPLC after enzymatic and acidic treatment.
Main Results:
- Rat gastric procolipase cDNA sequence is identical to pancreatic procolipase.
- High-fat diet led to decreased gastric procolipase expression.
- Enterostatin was detected in gastric juice, indicating cleavage of procolipase by pepsin and acid.
Conclusions:
- Gastric procolipase activation releases colipase and enterostatin.
- Gastric colipase may initiate fat digestion in the stomach.
- Gastric enterostatin might play a role in early satiety signaling.
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