Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Association of HAP1 isoforms with a unique cytoplasmic structure

S H Li1, C A Gutekunst, S M Hersch

  • 1Department of Genetics, Emory University School of Medicine, Atlanta, Georgia 30322, USA.

Journal of Neurochemistry
|November 3, 1998
PubMed
Summary

The study identifies unique cytoplasmic inclusions in rat neurons associated with HAP1 isoforms. HAP1-A is crucial for forming these structures, which may involve heteromultimers of HAP1-A and HAP1-B.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Severe oral and maxillofacial space infection in an infant caused by <i>Staphylococcus aureus</i>].

Zhonghua er ke za zhi = Chinese journal of pediatrics·2026
Same author

Effects of maternal peripartum supplementation with Schizochytrium oil on metabolic status, colostrum quality, and neonatal calf performance and immunity in dairy cows.

Journal of dairy science·2026
Same author

Integrative analysis of circRNA, miRNA, and mRNA profiles to reveal ceRNA regulation in geese ovarian development from the birth /laying to ceased periods.

Poultry science·2026
Same author

[A comparative analysis of diagnostic and therapeutic preferences for Graves' disease among Chinese and international endocrinologists].

Zhonghua yi xue za zhi·2025
Same author

[Impact of tumor circumferential location on prognosis in mid-low rectal cancer: a propensity- score-matched analysis].

Zhonghua wei chang wai ke za zhi = Chinese journal of gastrointestinal surgery·2025
Same author

Application and development of infrared technology in gas detection.

The Review of scientific instruments·2025

Area of Science:

  • Neuroscience
  • Cell Biology

Background:

  • HAP1 (Huntingtin Associated Protein 1) is a neural protein interacting with huntingtin, a protein linked to Huntington's disease.
  • Two HAP1 isoforms, HAP1-A and HAP1-B, exist with distinct C-terminal sequences.

Purpose of the Study:

  • To investigate the association of HAP1 isoforms with specific cellular structures in neurons.
  • To determine the role of HAP1 isoforms in the formation of neuronal cytoplasmic inclusions.

Main Methods:

  • Immunohistochemistry in rat brain neurons.
  • Cell line transfection studies (HEK 293 cells) with HAP1-A and HAP1-B cDNA.
  • Yeast two-hybrid assays to study protein self-association.

Main Results:

  • Both HAP1 isoforms associate with a unique, electron-dense cytoplasmic inclusion in rat neurons, resembling previously described structures like stigmoid bodies.

Related Experiment Videos

  • Transfection with HAP1-A cDNA induced similar inclusions, indicating its essential role in their formation.
  • Both HAP1-A and HAP1-B can self-associate, and their coexpression ratio regulates inclusion formation.

    • Conclusions:

    • HAP1-A is essential for the formation of HAP1-immunoreactive cytoplasmic inclusions in neurons.
    • These inclusions may be formed by heteromultimers of HAP1-A and HAP1-B.
    • The ratio of HAP1 isoforms influences the assembly of these neuronal structures.