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Relationship between the DNA content of human chromosomes and their involvement in radiation-induced structural
S Cigarrán1, L Barrios, J F Barquinero
1Dpt. Biologia Animal, Biologia Vegetal i Ecologia, Facultat de Ciències, Universitat Autònoma de Barcelona, Bellaterra, Spain.
Purpose:
To study the relationship between the DNA content of human chromosomes and their involvement in radiation-induced structural chromosome aberrations.
Material And Methods:
Human lymphocytes were cultured after exposure to 5 Gy of X-rays. FISH-painting was performed for all human chromosomes.
Results:
The results indicate that with the exception of chromosome 20, there was a good fit between the DNA content and the number of exchange-type aberrations and the number of breaks. However, there was a significant tendency for short chromosomes to be more affected than expected and for long chromosomes to be less affected than expected. A better fit was observed when, instead of DNA content, the surface area of the chromosome territories obtained from a spherical model was taken into consideration.
Conclusions:
The tendency for short chromosomes to be more involved and long chromosomes to be less involved in exchange-type aberrations could be related not only to their DNA content but also to their interphase territory surface area.