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Related Experiment Videos

Serum response factor is essential for mesoderm formation during mouse embryogenesis

S Arsenian1, B Weinhold, M Oelgeschläger

  • 1Institut für Molekularbiologie, Medizinische Hochschule Hannover, Carl-Neuberg-Strasse 1, 30625 Hannover, Germany.

The EMBO Journal
|November 3, 1998
PubMed
Summary
This summary is machine-generated.

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Serum response factor (SRF) is essential for mammalian mesoderm formation. SRF-deficient embryos exhibit severe gastrulation defects, highlighting its critical role in early development.

Area of Science:

  • Developmental Biology
  • Molecular Biology
  • Genetics

Background:

  • Serum response factor (SRF) is a conserved transcription factor regulating cellular responses to stimuli.
  • SRF is a target of the Ras/MAPK signaling network, influencing diverse cellular processes.
  • Previous SRF studies were limited to cultured cells, lacking in vivo multicellular organism data.

Purpose of the Study:

  • To investigate the function of SRF in a multicellular organism.
  • To determine the role of SRF in mammalian embryonic development and mesoderm formation.

Main Methods:

  • Generation of a null allele for SRF in mice (Srf -/-).
  • Analysis of SRF-deficient embryos for developmental defects and gene expression.
  • Assessment of SRF-regulated immediate early genes and muscle-specific genes.

Related Experiment Videos

Main Results:

  • SRF-deficient embryos display severe gastrulation defects and do not survive to term.
  • Absence of primitive streak and mesodermal cells in Srf -/- embryos.
  • Impaired expression of developmental markers (Bra, Bmp-2/4, Shh) and SRF-regulated genes (Egr-1, c-fos, alpha-Actin).

Conclusions:

  • SRF is identified as a novel and essential regulator of mammalian mesoderm formation.
  • Ras/MAPK signaling likely contributes to mesoderm induction in mammals, similar to amphibians.