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Related Experiment Videos

Interchromosomal recombination in Zea mays

W Hu1, M C Timmermans, J Messing

  • 1Waksman Institute, Rutgers, The State University of New Jersey, Piscataway, New Jersey 08855-0759, USA.

Genetics
|November 3, 1998
PubMed
Summary

A new maize allele was created through recombination between inbred lines, revealing significant sequence differences and a novel structure. This finding explains somatic recombination and suggests heritable new alleles can arise from chimeric chromosomes.

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Area of Science:

  • Genetics
  • Molecular Biology
  • Plant Science

Background:

  • Zein proteins are major storage proteins in maize endosperm.
  • Allelic variation at the 27-kD zein locus is crucial for understanding maize genetics.
  • Interactions between different maize inbred lines can lead to novel genetic structures.

Purpose of the Study:

  • To characterize a novel maize allele generated by interchromosomal recombination.
  • To investigate the structural differences between homologous chromosomes of maize inbred lines W64A and A188.
  • To explore the implications of these differences for recombination and allele evolution.

Main Methods:

  • Analysis of DNA sequences flanking a single crossover event between maize chromosomes.
  • Comparison of gene content and repetitive elements in homologous regions of W64A and A188.

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  • Identification of single base changes and structural variations, including insertions and deletions.

    • Main Results:

    • A new maize allele was generated via interchromosomal recombination, inserting ~11.8 kbp from W64A into A188.
    • Significant sequence divergence exists between the homologous regions, including a retrotransposon (Zeon-1) in W64A and a second zein gene copy in A188.
    • Recombination resulted in a 7.3-kb insertion and a 14-kb deletion relative to the original A188 allele, with 206 clustered base changes.

    Conclusions:

    • The structural heterogeneity of maize alleles can facilitate recombination despite sequence divergence.
    • The characterized allele structure provides a model for understanding somatic recombination events in maize hybrids.
    • Somatically derived chimeric chromosomes can be heritable, leading to the formation of new alleles and contributing to genetic diversity.