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A database of experimental results on globin gene expression
C Riemer1, A ElSherbini, N Stojanovic
1The Center for Gene Regulation, The Pennsylvania State University, University Park, Pennsylvania, 16802, USA.
Genomics
|November 4, 1998
Summary
A new database prototype, the Globin Gene Server, stores and queries gene expression data for mammalian beta-like globin genes. It facilitates comparative genomics by aligning DNA sequences across mammals.
Area of Science:
- Genomics
- Bioinformatics
- Molecular Biology
Background:
- Gene expression and regulation information is rapidly expanding.
- Complex experimental designs and data require specialized databases.
- Existing systems may not adequately store diverse gene expression results.
Purpose of the Study:
- To describe a prototype database for storing and querying mammalian beta-like globin gene expression data.
- To introduce novel query methods for accessing complex genetic information.
- To provide a framework for comparative genomics across mammalian species.
Main Methods:
- Developed a database with tables for DNA transfer experiments, protein-DNA binding, and DNase hypersensitive sites.
- Implemented nested data structures to manage complex experimental results.
- Established a common coordinate system through simultaneous DNA sequence alignment for cross-mammalian comparisons.
Main Results:
- A functional prototype database, the Globin Gene Server, was created.
- Multiple query methods are available for accessing stored gene expression data.
- The database supports comparative analysis of globin gene expression across mammals.
Conclusions:
- The Globin Gene Server provides a valuable resource for studying mammalian beta-like globin gene expression.
- The database design and query methods are adaptable to other genetic systems with available DNA sequence data.
- Interactive web access enhances the utility and accessibility of the genetic information.