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Sensitivity-enhanced detection of fast exchanging protons by an exchange-edited gradient HEHAHA-HSQC experiment
1Department of Biochemistry, University of Minnesota, Medical School, 4-225 Millard Hall, 435 Delaware Street S.E., Minneapolis, Minnesota, 55455, USA.
Abstract:
An experiment is presented which allows for the sensitivity-enhanced measurement of proton exchange rates in a HSQC type experiment. Instead of using INEPT type transfer of magnetization from protons to heteronuclei and vice versa, we have used heteronuclear Hartmann-Hahn transfer, which is known to have higher sensitivity in the presence of chemical exchange. Direct NOE's between NH protons and alpha-protons are suppressed by an exchange-editing step unless the alpha-resonances are degenerate with the water resonance. A comparison between the exchange-edited HEHAHA-HSQC and a standard exchange-edited HSQC experiment performed on the uniformly 15N-labeled staphylococcal nuclease H124L shows an enhancement of approximately 100% with the former experiment. A set of one-dimensional exchange-edited spectra of urea was used for evaluating the ability to extract exchange rates using the presented experiment.
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