Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Replication conrol: choreographing replication origins

J F Diffley1

  • 1ICRF Clare Hall Laboratories South Mimms, Hertfordshire, EN6 3LD, UK. J.Diffley@icrf.icnet.uk

Current Biology : CB
|November 4, 1998
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Coping with and recovering from hydroxyurea-induced replication fork arrest in budding yeast.

Cold Spring Harbor symposia on quantitative biology·2003
Same author

Mrc1 transduces signals of DNA replication stress to activate Rad53.

Nature cell biology·2001
Same author

MCM2-7 proteins are essential components of prereplicative complexes that accumulate cooperatively in the nucleus during G1-phase and are required to establish, but not maintain, the S-phase checkpoint.

Molecular biology of the cell·2001
Same author

Separate SCF(CDC4) recognition elements target Cdc6 for proteolysis in S phase and mitosis.

The EMBO journal·2001
Same author

Human origin recognition complex binds to the region of the latent origin of DNA replication of Epstein-Barr virus.

The EMBO journal·2001
Same author

Regulation of DNA replication fork progression through damaged DNA by the Mec1/Rad53 checkpoint.

Nature·2001
Same journal

A global response contributes to tissue size robustness upon local induction of apoptosis.

Current biology : CB·2026
Same journal

Prebilaterian origin of monoaminergic signaling.

Current biology : CB·2026
Same journal

CLASP-dependent microtubule stabilization generates microtubule-based protrusive forces during Drosophila epithelial morphogenesis.

Current biology : CB·2026
Same journal

Pigeons make slow, divergent eye movements during flight and large, convergent eye movements when landing.

Current biology : CB·2026
Same journal

Temperature signals drive grass secondary cell wall thickening.

Current biology : CB·2026
Same journal

Neuronal RNAi and oxygen-sensing circuit shape germline resilience to heat stress.

Current biology : CB·2026
See all related articles

Budding yeast replication origins activate during S phase via a set temporal program. S-phase-promoting cyclins help execute this by assembling a pre-initiation complex containing Cdc45 protein.

Area of Science:

  • Molecular Biology
  • Cell Cycle Regulation
  • Genetics

Background:

  • DNA replication is a fundamental process for cell division.
  • In budding yeast, replication origins are activated sequentially during S phase.
  • The precise temporal control of origin activation remains an area of active research.

Purpose of the Study:

  • To investigate the role of S-phase-promoting cyclins in the temporal control of DNA replication origin activation.
  • To elucidate the mechanism by which cyclins contribute to the assembly of the pre-initiation complex.

Main Methods:

  • Utilizing budding yeast (Saccharomyces cerevisiae) as a model organism.
  • Employing molecular biology techniques to study protein interactions and complex formation.
  • Analyzing the temporal activation of replication origins during the cell cycle.

Related Experiment Videos

Main Results:

  • Evidence suggests S-phase-promoting cyclins are key regulators of the temporal program for origin firing.
  • These cyclins facilitate the assembly of a pre-initiation complex essential for replication initiation.
  • The Cdc45 protein is identified as a crucial component of this cyclin-dependent complex.

Conclusions:

  • S-phase-promoting cyclins play a critical role in orchestrating the timing of DNA replication initiation in budding yeast.
  • The assembly of the pre-initiation complex, involving Cdc45, is a key step regulated by cyclins.
  • These findings provide insights into the molecular mechanisms governing the cell cycle and DNA replication.