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Oral Transmission of Listeria monocytogenes in Mice via Ingestion of Contaminated Food
Published on: May 6, 2013
A small outbreak of listeriosis associated with smoked mussels
M S Brett1, P Short, J McLauchlin
1Institute of Environmental Science and Research, Porirua, New Zealand. maggie.brett@esr.cri.nz
Abstract:
Two perinatal listeriosis cases in Auckland, New Zealand, which were diagnosed during November and December 1992 gave histories of consuming Brand X smoked mussels. Listeria monocytogenes was isolated from an unopened packet of mussels collected from the refrigerator of one of the cases. Cultures of L. monocytogenes from the two patients were indistinguishable from those recovered from the mussels by serogrouping and DNA macrorestriction analysis using pulsed-field gel electrophoresis (PFGE). PFGE analysis of isolates of an additional fifteen clinical cases caused by L. monocytogenes serogroup 1/2 which occurred in New Zealand during 1991 and 1992, revealed two more isolates with indistinguishable PFGE patterns. PFGE analysis of a further 222 L. monocytogenes serogroup 1/2 isolated in New Zealand from food and the environment did not reveal any more cultures with the Brand X PFGE type. A combination of serotyping, phage typing, DNA restriction fragment length polymorphism analysis (RFLP), cadmium and arsenic sensitivity testing, and PFGE analysis was used to subtype 38 isolates of L. monocytogenes. The isolates comprised: (a) the isolates from four patients; (b) 26 isolates from 15 packets of Brand X mussel products from retail sale, the processing factory, and the refrigerator of one of the patients; (c) seven isolates from environmental swabs taken in the processing factory; and (d) an isolate from Brand X mussel product from a wholesaler in the United Kingdom. The isolates from three of the clinical cases, 26 of the products and four from the factory environment were indistinguishable using all the subtyping systems. This is the first description from New Zealand of a link between cases of listeriosis, a contaminated food, and the food production environment which was microbiologically confirmed using a combination of subtyping methods for L. monocytogenes.
Insights
Two perinatal listeriosis cases were linked to Brand X smoked mussels in New Zealand. Microbiological analysis confirmed Listeria monocytogenes contamination in mussels and the food production environment.
Area of Science:
- Foodborne illness
- Microbiology
- Epidemiology
Background:
- Perinatal listeriosis poses significant risks.
- Brand X smoked mussels were identified as a potential food source in two cases.
- Listeria monocytogenes is a known foodborne pathogen.
Purpose of the Study:
- To investigate the link between perinatal listeriosis cases and Brand X smoked mussels.
- To identify the source of Listeria monocytogenes infection.
- To confirm the food production environment's role.
Main Methods:
- Isolating Listeria monocytogenes from patient and food samples.
- Utilizing serogrouping and DNA macrorestriction analysis (pulsed-field gel electrophoresis - PFGE).
- Employing a combination of subtyping methods: serotyping, phage typing, RFLP, and sensitivity testing.
Main Results:
- Listeria monocytogenes was isolated from patient samples and Brand X smoked mussels.
- PFGE analysis showed indistinguishable strains between patients, mussels, and the factory environment.
- Subtyping confirmed a link between clinical cases, contaminated mussels, and the processing factory.
Conclusions:
- This study provides the first microbiological confirmation in New Zealand of a foodborne listeriosis outbreak linked to a specific food product and its production environment.
- The findings highlight the importance of robust food safety measures in preventing listeriosis.
- Contaminated smoked mussels were identified as the likely source of infection.
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