Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Universal rules and idiosyncratic features in tRNA identity

R Giegé1, M Sissler, C Florentz

  • 1Unité Propre de Recherche 9002, 'Structure des Macromolécules Biologiques et Mécanismes de Reconnaissance', Scientifique, 15 rue René Descartes, F-67084, Strasbourg Cedex, France. giege@ibmc.u-strasbg.fr

Nucleic Acids Research
|November 4, 1998
PubMed
Summary

Transfer RNAs (tRNAs) use molecular signals to ensure accurate protein synthesis. These signals, often at the tRNA ends, guide specific amino acid attachment for genetic code fidelity.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Mitochondria: an organelle for life.

Biochimie·2014
Same author

Crystal quality and differential crystal-growth behaviour of three proteins crystallized in gel at high hydrostatic pressure.

Acta crystallographica. Section D, Biological crystallography·2005
Same author

Effects of macromolecular impurities and of crystallization method on the quality of eubacterial aspartyl-tRNA synthetase crystals.

Acta crystallographica. Section D, Biological crystallography·2005
Same author

Comparative analysis of thaumatin crystals grown on earth and in microgravity.

Acta crystallographica. Section D, Biological crystallography·2004
Same author

Human mitochondrial tRNAs in health and disease.

Cellular and molecular life sciences : CMLS·2003
Same author

From conventional crystallization to better crystals from space: a review on pilot crystallogenesis studies with aspartyl-tRNA synthetases.

Acta crystallographica. Section D, Biological crystallography·2002

Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • Accurate translation of the genetic code relies on transfer RNA (tRNA) identity.
  • tRNA identity dictates specific aminoacylation by cognate synthetases.

Purpose of the Study:

  • To survey molecular signals within tRNAs that determine specific aminoacylation.
  • To elucidate the mechanisms and rules governing tRNA identity.

Main Methods:

  • Review of existing literature on tRNA identity elements.
  • Analysis of molecular determinants in tRNA structure.
  • Examination of synthetase-tRNA interactions.

Main Results:

  • tRNA identity determinants are primarily located at the anticodon loop and amino acid accepting stem, but sometimes in the core.

Related Experiment Videos

  • Identity elements exhibit varied strengths and complex interplay (additive, cooperative, anti-cooperative).
  • Specific chemical groups on tRNA bases and ribose moieties mediate interactions with synthetases.

    • Conclusions:

    • tRNA identity rules are crucial for genetic code accuracy and are conserved evolutionarily.
    • Understanding these rules enables engineering of tRNAs with novel specificities.
    • tRNA architecture and anti-determinants play key roles in preventing misaminoacylation.