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Related Experiment Videos

A modified cell surface marker gene for transgenic animal studies

L Kieffer1, P B Kavathas

  • 1Department of Laboratory Medicine, Department of Genetics and Section of Immunobiology, Yale University,333 Cedar Street, PO Box 208035, New Haven, CT 06520-8035, USA.

Nucleic Acids Research
|November 4, 1998
PubMed
Summary

Researchers created a novel CD8 marker gene for transgenic animal studies. This modified gene allows for precise tracking of cell expression without unintended signaling, enhancing research accuracy.

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Area of Science:

  • Biotechnology
  • Molecular Biology
  • Immunology

Background:

  • Transgenic animal models are crucial for studying gene function and disease.
  • Developing reliable reporter genes is essential for accurate tracking of gene expression in vivo.
  • Existing marker genes may have limitations in specificity or signaling interference.

Purpose of the Study:

  • To develop a novel, non-signaling marker gene for use in transgenic animal studies.
  • To ensure the marker gene's expression can be reliably detected and quantified.
  • To provide a tool for precise monitoring of cell populations in complex biological systems.

Main Methods:

  • Engineered a CD8 cDNA with specific mutations to abolish its signaling function.
  • Linked the modified CD8 cDNA to a human growth hormone gene fragment.

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  • Utilized a CD2 enhancer to direct expression in target cell types within transgenic animals.
  • Employed monoclonal antibodies for detection via microscopy and flow cytometry.

    • Main Results:

    • Successfully generated a CD8 marker gene incapable of signaling through its known pathway.
    • Demonstrated appropriate cell-type-specific expression of the marker gene in transgenic animals.
    • Confirmed the utility of monoclonal antibodies for detecting the marker gene product.

    Conclusions:

    • The developed CD8 marker gene offers a reliable and specific tool for transgenic research.
    • This marker facilitates accurate monitoring of gene expression without confounding biological responses.
    • The engineered gene enhances the precision and scope of studies utilizing transgenic animal models.