Is there really an NPY Y3 receptor?

NPY is an abundant neuropeptide that is widely distributed throughout the central and peripheral nervous systems. Based on pharmacological and cloning data, there are believed to be six different types of NPY receptors. The Y3 receptor is the only one of the six that has not been cloned or well characterized. Y3 receptors have been shown to be important in the regulation of visceral afferents within the nucleus tractus solitarius (NTS). In the present experiments, we have compared the effects of NPY and various analogs on Ba2+ currents in acutely dissociated neurons from the NTS and the neighboring area postrema (AP). No neurons from either NTS or AP responded to [D-Trp32]NPY suggesting that these areas lack Y5 receptors. However, we found a group of NTS neurons that only responded to NPY and not PYY or any other analogs. This agonist profile corresponds to that described for NPY Y3 receptors. No AP neurons showed this type of agonist profile. Other neurons responded to a variety of NPY analogs indicating the presence of Y1, Y2, and Y4 receptors in both nuclei.