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Expression analysis of the autolysin gene (atl) of Staphylococcus aureus
1Lead Generation Research Laboratory, Tanabe Seiyaku Co., Ltd., Toda, Saitama, Japan. oshidata@genox.co.jp
Microbiology and Immunology
|November 5, 1998
Abstract:
The bifunctional autolysin gene (atl) of Staphylococcus aureus was transcribed into a 4.1-kb transcript. The transcription initiation site was located at an adenine residue 33-nt upstream from the putative atl start codon. Analysis using a promoter-reporter plasmid showed that promoter activity increased during the exponential growth phase. The Tn551 insertion site of the autolysis-deficient mutant S. aureus RUSAL2 was located in the putative catalytic region of the glucosaminidase domain.