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Quantitative Polymerase Chain Reaction (qPCR)-Based Rapid Diagnosis of Helicobacter pylori Infection and Antibiotic Resistance
06:40

Quantitative Polymerase Chain Reaction (qPCR)-Based Rapid Diagnosis of Helicobacter pylori Infection and Antibiotic Resistance

Published on: July 28, 2023

[A comparative analysis of diagnostic methods for Helicobacter pylori infection in children]

R Parejo Carranza1, F Olivares Miguel, H Escobar Castro

  • 1Servicio de Pediatria y Microbiología, Hospital Ramón y Cajal, Madrid.

Anales Espanoles De Pediatria
|November 6, 1998
PubMed

Insights

The carbon urea breath test is a highly accurate diagnostic tool for Helicobacter pylori (Hp) infection in children. This non-invasive test aids in selecting patients who require further endoscopic examination for Hp diagnosis.

Area of Science:

  • Pediatric Gastroenterology
  • Infectious Diseases
  • Diagnostic Medicine

Background:

  • Helicobacter pylori (Hp) infection is a common cause of gastrointestinal issues in children.
  • Accurate diagnosis is crucial for effective treatment and management.
  • Several diagnostic methods exist, each with varying accuracy and invasiveness.

Purpose of the Study:

  • To evaluate the diagnostic utility of five different tests for Helicobacter pylori (Hp) infection in pediatric patients.
  • To compare the sensitivity and specificity of these tests in a pediatric cohort.

Main Methods:

  • Sixty-five children with gastrointestinal symptoms underwent investigation for Hp infection.
  • Diagnostic tests included urease rapid test, histology, microbiological culture, IgG ELISA, and carbon urea breath test.
  • Endoscopic biopsies were analyzed, and breath tests were performed on a subset of patients.

Main Results:

  • Helicobacter pylori (Hp) infection was confirmed in 53 children.
  • The carbon urea breath test demonstrated 96% positivity in infected patients and 100% negativity in uninfected patients.
  • Urease rapid test and culture showed high positivity rates (96% and 89% respectively), but with some false positives.
  • IgG antibodies were detected in 67% of infected children.

Conclusions:

  • The carbon urea breath test is a highly sensitive and specific non-invasive method for diagnosing Helicobacter pylori (Hp) infection in children.
  • This test can effectively identify children who would benefit from endoscopy, optimizing diagnostic pathways.
Abstract

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