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Ureaplasma urealyticum reduces motility and induces membrane alterations in human spermatozoa
R Núñez-Calonge1, P Caballero, C Redondo
1Department of Gynecology, Ramón y Cajal Hospital, Institute Nacional of Health, Madrid, Spain.
Abstract:
The in-vitro effects of several concentrations of Ureaplasma urealyticum on the motility, membrane integrity and morphology of washed spermatozoa from healthy donors were studied. A significant reduction in sperm motility and signs of membrane alteration, directly related to U.urealyticum concentration and contact time were observed. Scanning electron microscopy examination showed masses of U. urealyticum attached to the head and middle piece of some of deformed spermatozoa. It is suggested that U.urealyticum is involved in sperm changes leading to male infertility, particularly when there is heavy U. urealyticum colonization or specific infections with this microorganism.
Insights
Ureaplasma urealyticum significantly reduces sperm motility and damages sperm membranes in vitro. This microorganism may contribute to male infertility, especially with heavy colonization.
Area of Science:
- Reproductive biology
- Microbiology
- Andrology
Background:
- Ureaplasma urealyticum is a common bacterium found in the urogenital tract.
- Its role in male infertility is not fully understood, necessitating further investigation into its direct effects on sperm.
Purpose of the Study:
- To investigate the in vitro effects of Ureaplasma urealyticum on human sperm parameters.
- To determine the relationship between U. urealyticum concentration, contact time, and sperm damage.
Main Methods:
- Washed spermatozoa from healthy donors were exposed to varying concentrations of Ureaplasma urealyticum.
- Sperm motility, membrane integrity, and morphology were assessed.
- Scanning electron microscopy was used to visualize bacterial attachment to spermatozoa.
Main Results:
- A significant decrease in sperm motility was observed with increasing U. urealyticum concentration and exposure time.
- Evidence of sperm membrane damage was directly correlated with bacterial load and duration of contact.
- Scanning electron microscopy revealed U. urealyticum adhering to deformed spermatozoa, particularly on the head and midpiece.
Conclusions:
- Ureaplasma urealyticum negatively impacts sperm motility, membrane integrity, and morphology in vitro.
- These findings suggest a potential causal link between U. urealyticum infection/colonization and male infertility.
- Further research is warranted to elucidate the precise mechanisms of U. urealyticum-induced sperm dysfunction.