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Culture of Macrophage Colony-stimulating Factor Differentiated Human Monocyte-derived Macrophages
06:46

Culture of Macrophage Colony-stimulating Factor Differentiated Human Monocyte-derived Macrophages

Published on: June 30, 2016

A novel macrophage actin-associated protein (MAYP) is tyrosine-phosphorylated following colony stimulating factor-1

Y G Yeung1, S Soldera, E R Stanley

  • 1Department of Developmental and Molecular Biology, Albert Einstein College of Medicine, Bronx, New York 10461, USA.

The Journal of Biological Chemistry
|November 7, 1998
PubMed

Insights

A novel protein, macrophage actin-associated tyrosine-phosphorylated protein (MAYP), is rapidly tyrosine-phosphorylated in macrophages upon colony-stimulating factor-1 (CSF-1) stimulation. MAYP associates with F-actin and is likely involved in CSF-1-induced actin cytoskeleton reorganization.

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Biochemistry

Background:

  • Colony-stimulating factor-1 (CSF-1) is crucial for macrophage survival, proliferation, and differentiation.
  • CSF-1 signaling regulates cellular functions, including actin cytoskeleton dynamics.
  • Tyrosine phosphorylation plays a key role in signal transduction pathways in macrophages.

Purpose of the Study:

  • To identify and characterize novel proteins involved in CSF-1 signaling in macrophages.
  • To investigate the role of tyrosine-phosphorylated proteins in CSF-1-induced actin reorganization.
  • To elucidate the function of a newly identified 37-kDa tyrosine-phosphorylated protein.

Main Methods:

  • Purification of the 37-kDa protein (pp37) using anti-phosphotyrosine affinity chromatography and HPLC.
  • Peptide sequencing and EST analysis to identify the protein.
  • cDNA cloning to obtain the full-length pp37 sequence.
  • Expression analysis in different tissues and cell lines.
  • Subcellular localization studies and assessment of CSF-1-induced translocation.

Main Results:

  • A 37-kDa cytoplasmic protein (pp37) was identified and purified.
  • pp37 shares sequence similarity with PSTPIP/CDC15 family substrates and contains FCH and actin-binding domains.
  • pp37 is selectively expressed in macrophages and associated with F-actin in the cytosol.
  • CSF-1 stimulation increases the association of pp37 with the cytoskeletal fraction.
  • The protein was named macrophage actin-associated tyrosine-phosphorylated protein (MAYP).

Conclusions:

  • MAYP is the major F-actin-associated protein tyrosine-phosphorylated in macrophages.
  • MAYP is a novel substrate in the CSF-1 signaling pathway.
  • MAYP likely plays a significant role in regulating CSF-1-induced actin cytoskeleton remodeling in macrophages.

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