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Sequence of murine CDC10 cDNA, gene organization and expression analysis
1Laboratoire de Génétique Biochimique et de Cytogénétique, INRA, 78352 Jouy-en-Josas Cedex, France.
Biochimica Et Biophysica Acta
|November 7, 1998
Summary
Researchers sequenced the murine CDC10 cDNA, identifying a gene encoding a GTP-binding protein highly similar to human CDC10. This gene is expressed ubiquitously across tissues, suggesting a fundamental cellular role.
Area of Science:
- Molecular Biology
- Genetics
- Biochemistry
Background:
- The CDC10 gene plays a crucial role in cell division and is conserved across species.
- Understanding the murine CDC10 gene provides insights into mammalian cell cycle regulation.
Purpose of the Study:
- To report the full sequence of the murine CDC10 cDNA.
- To characterize the gene structure and expression pattern of murine CDC10.
Main Methods:
- Sequencing of the 2391 bp murine CDC10 cDNA.
- Northern blot analysis to assess gene expression across tissues.
Main Results:
- The murine CDC10 gene comprises 13 exons.
- It encodes a potential 417/446 amino acid GTP-binding protein.
- Northern analysis revealed ubiquitous gene expression, consistent with its promoter structure.
Conclusions:
- The murine CDC10 gene is highly similar to its human counterpart.
- Ubiquitous expression suggests a fundamental role in cellular processes in mice.