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Related Experiment Videos

Round spermatid transfer and embryo development

I Sasagawa1, O Ichiyanagi, H Yazawa

  • 1Department of Urology, Yamagata University School of Medicine, Japan. isasaga@med.id.yamagata-u.ac.jp

Archives of Andrology
|November 7, 1998
PubMed
Summary

Assisted reproductive technology enables pregnancy without sperm using round spermatid nuclei. These nuclei can fertilize oocytes, leading to normal offspring, offering hope for infertility treatments.

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Area of Science:

  • Reproductive Biology
  • Developmental Biology
  • Genetics

Background:

  • Assisted reproductive technology (ART) has advanced significantly.
  • Pregnancy is achievable without traditional spermatozoa.
  • The nucleus of round spermatids offers a potential alternative.

Purpose of the Study:

  • To investigate the potential of round spermatid nuclei as sperm substitutes.
  • To explore the developmental capacity of oocytes fertilized with spermatid nuclei.
  • To assess the feasibility of using immature germ cells for treating azoospermia.

Main Methods:

  • Nuclei from mouse round spermatids (from cryptorchid and prepubertal testes) were injected into oocytes.
  • Oocyte activation was induced using electrostimulation and oscillogen injection.

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  • Nuclei from mouse primary spermatocytes were also injected into oocytes.

    • Main Results:

    • Round spermatid nuclei successfully fertilized oocytes, leading to normal embryonic development and live offspring in mice.
    • Spermatid nucleus development into a pronucleus required artificial oocyte activation.
    • Injected primary spermatocyte nuclei underwent meiosis, formed embryos, and resulted in live young.

    Conclusions:

    • Round spermatid nuclei can function as effective sperm substitutes in mice.
    • Artificial oocyte activation is crucial for spermatid nucleus development.
    • Intracytoplasmic injection of immature male germ cells shows promise for treating azoospermia due to maturation arrest.