Purification of carp (Cyprinus carpio) hepatopancreatic lactate dehydrogenase (EC 1.1.1.27)

Lactate dehydrogenase (LDH, EC 1.1.1.27) was isolated in 86% purity from carp (Cyprinus carpio) hepatopancreas using agarose T-gel (mercaptoethanol bound to agarose via divinylsulphone) affinity chromatography medium and Iontosorb phenyl hydrophobic chromatography medium. Gel filtration chromatography of the LDH obtained shows molecular weight to be 26,900 Da.