Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Elastic protein-based polymers in soft tissue augmentation and generation

D W Urry1, A Pattanaik, J Xu

  • 1Bioelastic Research, Ltd., OADI Technology Center, Birmingham, AL 35211-6912, USA.

Journal of Biomaterials Science. Polymer Edition
|November 7, 1998
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Expression of an environmentally friendly synthetic protein-based polymer gene in transgenic tobacco plants.

Plant cell reports·2013
Same author

Changing trends in dietetics.

Ancient science of life·2012
Same author

Skin concentrations of thromboxane synthetase inhibitor after topical application with bioelastic membrane.

Journal of veterinary pharmacology and therapeutics·2004
Same author

Mechanics of elastin: molecular mechanism of biological elasticity and its relationship to contraction.

Journal of muscle research and cell motility·2003
Same author

Elastin: a representative ideal protein elastomer.

Philosophical transactions of the Royal Society of London. Series B, Biological sciences·2002
Same author

Elastomeric polypentapeptides cross-linked into matrixes and fibers.

Biomacromolecules·2001

Researchers developed novel elastic protein-based polymers for soft tissue augmentation. One polymer variant, incorporating a cell attachment sequence, successfully promoted tissue regeneration, offering a promising alternative to current treatments for urinary incontinence.

Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Regenerative Medicine

Background:

  • Current soft tissue augmentation materials, like Contigen, can result in scar tissue formation.
  • Elastic protein-based polymers offer potential for long-lasting tissue augmentation.
  • Preclinical models are crucial for evaluating biomaterials for soft tissue repair.

Purpose of the Study:

  • To evaluate the tissue response to novel elastic protein-based polymers as subcutaneous implants.
  • To assess the potential of these polymers for soft tissue augmentation and urinary incontinence correction.
  • To investigate the impact of specific sequence modifications on polymer-induced tissue regeneration.

Main Methods:

  • Recombinant DNA technology was used to produce six elastic protein-based polymers, including variations of polymer I ((GVGVP)251).

Related Experiment Videos

  • Polymers were purified to biomaterial standards and injected as subcutaneous implants in guinea pigs.
  • Tissue responses were analyzed at 2 and 4 weeks post-implantation, focusing on cellular infiltration, capsule formation, and matrix deposition.

    • Main Results:

    • Polymer I acted as a bulking agent with fibrous capsule formation.
    • Inclusion of a chemoattractant sequence (GVGVAP)8 led to macrophage infiltration.
    • Addition of a cell attachment sequence (GRGDSP) to polymer I resulted in significant tissue generation, including collagen, elastic fibers, and angiogenesis.

    Conclusions:

    • The elastic protein-based polymer with the GRGDSP sequence demonstrated promising tissue regenerative properties.
    • This engineered polymer may offer a long-lasting solution for soft tissue augmentation, potentially avoiding scar tissue formation.
    • Further studies are needed to confirm the long-term efficacy and safety of these novel biomaterials.