Rapid postadsorptive changes in fibrinogen adsorbed from plasma to segmented polyurethanes

Area of Science:

• Biomaterials Science
• Surface Chemistry
• Hematology

Background:

• Fibrinogen adsorption on biomaterials is critical for platelet interactions and blood clotting.
• Previous studies showed fibrinogen adsorbed from buffer becomes non-displaceable in plasma.
• Understanding fibrinogen behavior from plasma is essential for biomaterial development.

Purpose of the Study:

• To characterize the displaceability of fibrinogen adsorbed directly from blood plasma onto various surfaces.
• To investigate the influence of adsorption time and surface type on fibrinogen transitions.
• To compare fibrinogen behavior when adsorbed from plasma versus buffer.

Main Methods:

• Fibrinogen adsorption from 1% plasma to glass, silicone rubber, and five polyurethanes for 1-64 minutes.
• Transfer of surfaces to 100% plasma without labeled fibrinogen to measure displacement.
• Quantification of adsorbed fibrinogen before and after transfer to assess resistance.

Main Results:

• Fibrinogen adsorbed from plasma showed increased resistance to displacement with longer adsorption times.
• The rate of increased resistance varied significantly across different surface types.
• These surface-dependent transitions occurred rapidly.

Conclusions:

• Fibrinogen adsorbed from blood plasma undergoes rapid, surface-dependent transitions.
• The observed transitions are similar to those previously seen for fibrinogen adsorbed from buffer.
• These findings highlight the importance of surface properties in modulating fibrinogen behavior in blood contact applications.