Ca2+ regulation by the Na(+)-Ca2+ exchanger in retinal horizontal cells depolarized by L-glutamate

This study is concerned with regulation of the intracellular Ca2+ concentration ([Ca2+]i) of horizontal cells isolated from cyprinid fish retinae, with the main emphasis on the role of the (Na+)-Ca2+ exchanger. An inward current was blocked by Ca2+ (4 mM) during prolonged (> 1 h) depolarization by L-glutamate (100 microM) in the whole-cell voltage-clamp configuration, suggesting the persistent activation of voltage-gated Ca2+ channels. This (Co2+)-sensitive current was absent when extracellular Na+ was replaced by Li+ to suppress (Na+)-Ca2+ exchange. Measurement of [Ca2+]i using the Fura-2 ratiometric method gave the following results. (1) L-Glutamate (100 microM) caused [Ca2+]i to increase from the resting level of 75.4+/-36.8 nM (mean +/-S.D., n = 11) to the maximum level (2.2+/-1.4 microM, n = 11) within 15 s and then to decrease to a steady level of 0.59+/-0.23 microM (n = 11). (2) Nifedipine (100 microM) lowered the L-glutamate-induced steady [Ca2+]i level, which was still higher than the resting level. (3) L-Glutamate caused [Ca2+]i to increase even after blockading the voltage-gated Ca2+ channels by nifedipine or by clamping the membrane voltage at -55 mV. (4) (Na+)-free superfusate elevated the L-glutamate-induced steady [Ca2+]i level. (5) The time course of the [Ca2+]i decrease from the L-glutamate-induced steady level to the resting level was prolonged in the (Na+)-free superfusate. These results suggest that the (Na+)-Ca2+ exchanger extrudes intracellular Ca2+ to maintain a low [Ca2+]i level by counteracting the continuous Ca2+ influx through the voltage-gated Ca2+ channels and glutamate-gated channels when horizontal cells in situ are tonically depolarized by L-glutamate released from the photoreceptors. The (Na+)-Ca2+ exchange current isolated by a voltage-clamp experiment depends exponentially on the membrane potential.