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Nontoxigenic Vibrio cholerae O1 intestinal pathology in adult mice
M Moyenuddin1, R Weiss, I K Wachsmuth
1Georgia State University, Laboratory of Biological Sciences, Atlanta 30202, USA.
Abstract:
The intestinal pathology caused by nontoxigenic Vibrio cholerae O1 was examined in the sealed adult mouse (SAM) model. Histologic examination demonstrated that a nontoxigenic V. cholerae O1 strain that elicited maximum fluid accumulation (FA) in the small intestine of adult mice caused damages to the villi and necrosis of lymphoid elements within solitary submucosal lymphoid nodules in the Peyer's patches. Challenge of mice with a strain that did not elicit intestinal FA produced none of the above tissue responses. Increased FA activity, intestinal alterations, and tissue pathology caused by the nontoxigenic V. cholerae O1 indicate its pathogenic potential.
Insights
Nontoxigenic Vibrio cholerae O1 strains can cause intestinal damage and pathology in mice. These findings highlight the pathogenic potential of these bacteria, even without toxin production.
Area of Science:
- Microbiology
- Pathology
- Immunology
Background:
- Nontoxigenic Vibrio cholerae O1 strains are generally considered less virulent.
- Understanding the pathogenic mechanisms of these strains is crucial for public health.
Purpose of the Study:
- To investigate the intestinal pathology induced by nontoxigenic Vibrio cholerae O1 in the sealed adult mouse (SAM) model.
- To determine if nontoxigenic strains possess pathogenic potential.
Main Methods:
- Utilized the sealed adult mouse (SAM) model for infection studies.
- Performed histologic examination of intestinal tissues post-challenge.
- Assessed fluid accumulation (FA) in the small intestine.
Main Results:
- A nontoxigenic V. cholerae O1 strain eliciting maximum FA caused villi damage and lymphoid necrosis in Peyer's patches.
- Mice challenged with a non-FA-eliciting strain showed no significant tissue alterations.
- Increased FA activity correlated with observed intestinal alterations and pathology.
Conclusions:
- Nontoxigenic Vibrio cholerae O1 can induce significant intestinal pathology.
- The observed intestinal alterations and tissue damage indicate pathogenic potential.
- The SAM model is effective for studying Vibrio cholerae O1 pathogenesis.