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Binary oscillatory cross-flow electrophoresis: theory and experiments

R F Molloy1, D T Leighton

  • 1Department of Chemical Engineering University of Notre Dame Notre Dame, Indiana 46556, USA.

Journal of Pharmaceutical Sciences
|November 13, 1998
PubMed
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Binary Oscillatory Cross-flow Electrophoresis (BOCE) separates charged proteins using electric fields and fluid flow. This novel technique achieved high purity and throughput in experimental protein separations.

Area of Science:

  • Biophysics
  • Analytical Chemistry
  • Biotechnology

Background:

  • Electrophoretic separation techniques are crucial for analyzing charged biomolecules.
  • Existing methods face challenges in achieving high throughput and purity.
  • Novel approaches are needed to enhance protein separation efficiency.

Purpose of the Study:

  • To introduce and describe a new electrophoretic separation technique called Binary Oscillatory Cross-flow Electrophoresis (BOCE).
  • To investigate the principles and effectiveness of BOCE for separating charged protein species.
  • To provide a theoretical and experimental validation of the BOCE technique.

Main Methods:

  • Utilizing an oscillatory electric field and a transverse oscillatory shear flow within a rectangular channel.

Related Experiment Videos

  • Developing an analytic model to predict solute convective velocity based on electrophoretic mobility and flow parameters.
  • Conducting numerical simulations incorporating diffusion to analyze concentration profiles.
  • Performing experimental separations using a model protein system (BSA and BHb).

    • Main Results:

    • The BOCE technique effectively separates charged protein species by creating an active binary filter.
    • Theoretical predictions of solute convective velocity were supported by numerical simulations and experimental data.
    • Experimental runs achieved a solute throughput of 37 mg/h of 92% pure bovine serum albumen (BSA) in a 3 mL cell.

    Conclusions:

    • Binary Oscillatory Cross-flow Electrophoresis (BOCE) is a promising novel technique for efficient protein separation.
    • The developed analytic model accurately predicts separation behavior.
    • BOCE demonstrates potential for high-throughput and high-purity separation of charged biomolecules.