[Experimental study on free grafting of fat particles]

Forty-eight rats were used to study the change in fat tissue after grafting. In the experimental group, the fat tissue to be grafted was treated with insulin. Evaluation was made by graft measurement, ink injection, light microscopic and electronic microscopic observations. The results showed that the volume of the grafted fat decreased obviously and there was no significant difference between the experimental and the control groups in the change of volume of the grafted fat tissue. At the early stage of grafting, the grafted fat showed ischemia. The adipocytes released lipid and dedifferentiated to preadipocytes. After revascularization, the preadipocytes began to absorb lipid and became mature adipocytes. The structure of the grafted fat was almost normal at 6 months after grafting. The formation of the cytoskeletal filament, found by ultrastructural study, was closely related with the synthesis of lipid.