[Detection of poliovirus by digoxigenin-labeled cDNA probe prepared by PCR technique]

A study including reverse transcribed cDNAs of Sabin I, II, III virus used as templates and digoxigenin-labeled dUTP added to the reaction solution was carried out. After amplification, digoxigenin-labeled poliovirus cDNA probe was prepared by this PCR technique. Results showed that the direct preparation of digoxigenin-labeled poliovirus cDNA probe by PCR is a convenient and rapid method with a high labeling rate. Compared with neutralization test, the probe has the advantages of more rapid, more sensitive and more specific for poliovirus typing.