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[Experimental treatment of rhubarb on mesangio-proliferative glomerulonephritis in rats]
Abstract:
Accumulation of extracellular matrix is a prominent feature of progressive glomerulonephritis (GN). Previously, we have shown that Emodin, an important component of Rhubarb, inhibits the fibronectin (FN) production of cultured mesangial cells. We now provide evidence of the same effect of Rhubarb in immune complex GN induced in rats by injection of anti-thymocyte serum (ATS). Rhubarb ameliorated mesangial matrix expansion on day 7 after ATS administration. Decreased FN level and IL-1 activity were found in the supernatant of cultured glomeruli from Rhubarb-treated animal at the 7th day. Glomerular tissue FN fluorescence staining was also much weaker than that of the untreated rats. These studies demonstrated that Rhubarb-treated anti-Thy-1 animal model should develop significantly less matrix expansion. Rhubarb also inhibited synthesis and secretion of fibronectin, an important component of mesangial extracellular matrix. Decreased IL-1 activity might be involved in the therapeutic effect of Rhubarb on mesangioproliferative glomerulonephritis.
Insights
Rhubarb, a component of Emodin, reduces extracellular matrix buildup in progressive glomerulonephritis (GN). This study shows Rhubarb ameliorates mesangial matrix expansion and fibronectin production in an animal model of GN.
Area of Science:
- Nephrology
- Pharmacology
- Immunology
Context:
- Progressive glomerulonephritis (GN) is characterized by extracellular matrix accumulation.
- Emodin from Rhubarb previously showed inhibition of fibronectin (FN) in cultured mesangial cells.
Purpose:
- To investigate Rhubarb's effect on immune complex GN in rats.
- To evaluate Rhubarb's impact on mesangial matrix expansion and fibronectin levels.
Summary:
- Rhubarb administration ameliorated mesangial matrix expansion in an anti-thymocyte serum (ATS)-induced GN rat model.
- Reduced fibronectin levels and IL-1 activity were observed in glomeruli from Rhubarb-treated rats.
- Rhubarb inhibited fibronectin synthesis and secretion, potentially via decreased IL-1 activity.
Impact:
- Rhubarb demonstrates therapeutic potential for mesangioproliferative glomerulonephritis.
- The findings support Rhubarb's role in managing kidney fibrosis.
- This study highlights a potential natural compound for treating GN.