Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Human DNA polymerase beta recognizes single-stranded DNA using two different binding modes

S Rajendran1, M J Jezewska, W Bujalowski

  • 1Department of Human Biological Chemistry and Genetics, Sealy Center for Structural Biology, The University of Texas Medical Branch at Galveston, Galveston, Texas 77555-1053, USA.

The Journal of Biological Chemistry
|November 13, 1998
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Long-term trends in heavy metal contamination of marine sediments in the Arabian Gulf: A meta-analysis.

Environmental monitoring and assessment·2025
Same author

Interactions between descending and spinal circuits on motor output.

Experimental neurology·2025
Same author

Comment on: "Single versus double injection costoclavicular block: non-inferiority trial".

Revista espanola de anestesiologia y reanimacion..·2024
Same author

Do we consent to change? An evaluation of oral and maxillofacial outpatient department dentoalveolar consent forms.

The British journal of oral & maxillofacial surgery·2024
Same author

The Normal Static Two-Point Discrimination in the Palmar Aspect of Hand in Adults and Children in a Sample Indian Population.

Indian journal of plastic surgery : official publication of the Association of Plastic Surgeons of India·2024
Same author

Short-Term Memory Deficit Associates with miR-153-3p Upregulation in the Hippocampus of Middle-Aged Mice.

Molecular neurobiology·2023
Same journal

Isotope-Edited ESEEM: A New Method for Probing Copper Binding Sites in Neurodegenerative Proteins.

The Journal of biological chemistry·2026
Same journal

Introduction to the Thematic Review Series on Intracellular Protein Degradation. The ubiquitous biology of intracellular protein degradation: a tribute to Alfred L. ("Fred") Goldberg.

The Journal of biological chemistry·2026
Same journal

Correction: Aromatic residue-rich amino-terminal segments of temporin L self-assemble into collagen-mimetic peptides with cell-adhesion properties.

The Journal of biological chemistry·2026
Same journal

YhbO is a DJ-1 family glyoxalase and α-oxoaldehyde hydratase that confers resistance to reactive carbonyl stress (112).

The Journal of biological chemistry·2026
Same journal

ARMH3 acts as a central scaffold at the Golgi/TGN through interactions with Arl5, GBF1, and PI4KB.

The Journal of biological chemistry·2026
Same journal

PAX8 controls proximal tubule epithelial identity and stress response through epigenetic modification of distal regulatory elements.

The Journal of biological chemistry·2026
See all related articles

Human DNA polymerase beta (pol beta) exhibits two distinct binding modes with single-stranded DNA (ssDNA). These modes involve either 16 or 5 occluded nucleotides, impacting the enzyme's functional mechanisms.

Area of Science:

  • Molecular Biology
  • Biochemistry
  • Genetics

Background:

  • DNA polymerase beta (pol beta) is crucial for DNA repair pathways.
  • Understanding pol beta's interaction with single-stranded DNA (ssDNA) is key to elucidating its function.

Purpose of the Study:

  • To investigate the binding modes of human pol beta to ssDNA.
  • To characterize the stoichiometry and affinity of pol beta-ssDNA interactions.

Main Methods:

  • Quantitative fluorescence titration using poly(dA) etheno-derivative (poly(depsilonA)).
  • Analysis using statistical thermodynamic models accounting for cooperative binding and site overlap.

Main Results:

  • Human pol beta binds ssDNA in two distinct modes: a high-affinity mode (pol beta)16 with 16 occluded nucleotides, and a lower-affinity mode (pol beta)5 with 5 occluded nucleotides.

Related Experiment Videos

  • Binding affinity and stoichiometry were determined through fluorescence increase as a function of enzyme density.

    • Conclusions:

    • Human pol beta utilizes at least two different binding modes when interacting with ssDNA.
    • These distinct binding modes have significant implications for mechanistic models of human pol beta function in DNA repair and replication.