Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Overlapping 3'-end formation signals and ARS elements: tightly linked but functionally separable

C Magrath1, K Lund, C A Miller

  • 1Tulane University, Interdisciplinary Program in Molecular and Cell Biology, New Orleans, LA, USA.

Gene
|November 14, 1998
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Developing core indicators for identifying people at risk of delayed heart failure diagnosis.

BMC primary care·2025
Same author

Long-term comparison and performance study of consumer grade electronic radon integrating monitors.

Journal of radiological protection : official journal of the Society for Radiological Protection·2024
Same author

DUAL-PARTICLE DOSEMETER BASED ON ORGANIC SCINTILLATOR.

Radiation protection dosimetry·2020
Same author

A Framework for Climate Change-Related Research to Inform Environmental Protection.

Environmental management·2019
Same author

Preparation and Antibacterial Properties of Silver-Doped Nanoscale Hydroxyapatite Pastes for Bone Repair and Augmentation.

Journal of biomedical nanotechnology·2019
Same author

ClonEvol: clonal ordering and visualization in cancer sequencing.

Annals of oncology : official journal of the European Society for Medical Oncology·2017

Autonomous replicating sequences (ARSs) and 3'-end formation signals in Saccharomyces cerevisiae are not functionally interdependent. High transcription through ARSs, however, impairs plasmid replication, suggesting genome structure influences their association.

Area of Science:

  • Molecular Biology
  • Genetics
  • Yeast Biology

Background:

  • Autonomous replicating sequences (ARSs) in Saccharomyces cerevisiae often overlap with 3'-end formation signals.
  • Previous studies showed that inactivated ARSs do not affect 3'-end formation, indicating replication function is not essential for termination.

Purpose of the Study:

  • To investigate whether 3'-end formation signals are crucial for ARS replication function.
  • To determine the impact of transcription readthrough on ARS replication efficiency.

Main Methods:

  • Point mutations were introduced into ARS305 to increase readthrough of 3'-end formation signals.
  • Plasmid replication efficiency was assessed using stability assays under varying transcription conditions.

Main Results:

Related Experiment Videos

  • Mutations affecting 3'-end formation did not alter plasmid replication efficiency when transcription was absent or highly induced.
  • High-level transcription through ARSs significantly increased plasmid loss rates for both wild-type and mutated terminators.
  • The native 3'-end formation signal could not protect ARS replication function under strong transcriptional interference.

Conclusions:

  • 3'-End formation signals do not appear to be functionally interdependent with ARS replication in Saccharomyces cerevisiae.
  • The close proximity of transcription terminators and ARSs in the compact yeast genome may be driven by genomic organization rather than functional necessity.
  • Transcriptional interference poses a significant challenge to ARS function, highlighting the importance of regulatory mechanisms in dense genomes.