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Peptide-based OspC enzyme-linked immunosorbent assay for serodiagnosis of Lyme borreliosis

M J Mathiesen1, M Christiansen, K Hansen

  • 1Department of Clinical Biochemistry, Statens Serum Institut, Copenhagen, Denmark. mth@ssi.dk

Journal of Clinical Microbiology
|November 18, 1998
PubMed

Keywords:
Lyme disease diagnosticsOspC ELISAIgM antibody detectionBorrelia burgdorferiserological testing

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Enzyme-Linked Immunosorbent Assay01:33

Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or quantified.