Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Scratching the (cell) surface: cytokine engineering for improved ligand/receptor trafficking dynamics

D A Lauffenburger1, E M Fallon, J M Haugh

  • 1Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge 02139, USA. lauffen@mit.edu

Chemistry & Biology
|November 18, 1998
PubMed
Summary

Engineered cytokines show enhanced cellular function stimulation. Researchers are exploring protein engineering and receptor studies to identify optimal cytokine properties for improved biological responses, as binding affinity alone is insufficient.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Subdural extravasation of crystalloids and blood products through a scalp peripheral intravenous catheter into the subdural space of a neonate on veno-arterial extracorporeal membrane oxygenation.

Journal of neonatal-perinatal medicine·2021
Same author

Autosomal Dominant Gene Negative Frontotemporal Dementia-Think of SCA17.

Cerebellum (London, England)·2019
Same author

GPS for QSP: A Summary of the ACoP6 Symposium on Quantitative Systems Pharmacology and a Stage for Near-Term Efforts in the Field.

CPT: pharmacometrics & systems pharmacology·2016
Same author

Systematic Analysis of Quantitative Logic Model Ensembles Predicts Drug Combination Effects on Cell Signaling Networks.

CPT: pharmacometrics & systems pharmacology·2016
Same author

Distinct genital tract HIV-specific antibody profiles associated with tenofovir gel.

Mucosal immunology·2016
Same author

Distinct genital tract HIV-specific antibody profiles associated with tenofovir gel.

Mucosal immunology·2016

Area of Science:

  • Immunology and Protein Engineering
  • Cellular Biology and Signaling Pathways

Background:

  • Cytokines are crucial signaling molecules that regulate cellular functions.
  • Engineering cytokines for enhanced potency is a key area in therapeutic development.
  • Receptor-binding affinity is a common metric for cytokine efficacy but doesn't always predict biological outcome.

Purpose of the Study:

  • To investigate the relationship between engineered cytokine properties and cellular response.
  • To identify specific ligand-receptor binding characteristics that correlate with improved biological activity.
  • To bridge protein engineering with cellular trafficking and signaling studies for better cytokine design.

Main Methods:

  • Utilizing protein engineering techniques to modify cytokine structure.

Related Experiment Videos

  • Conducting studies on receptor trafficking dynamics.
  • Analyzing cytokine-mediated cellular signaling pathways.
  • Correlating binding affinity with functional cellular responses.

    • Main Results:

    • Receptor-binding affinity alone does not consistently predict enhanced biological response in engineered cytokines.
    • Specific protein-engineering modifications may influence receptor interactions and downstream signaling.
    • Understanding receptor trafficking and signaling is essential for optimizing cytokine function.

    Conclusions:

    • Optimizing engineered cytokines requires a multifaceted approach beyond simple binding affinity.
    • Combining protein engineering with cellular biology insights can guide the development of more effective cytokine therapeutics.
    • Further research into ligand-receptor dynamics is needed to identify key properties for improved cytokine potency.