Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Videos

New results using Laue diffraction and time-resolved crystallography

¹Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA. bstoddar@fred.fhcrc.org

Current Opinion in Structural Biology

|November 18, 1998

Summary

This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Hit-and-run programming of therapeutic cytoreagents using mRNA nanocarriers.

Nature communications·2017

Same author

Crystallization and preliminary X-ray analysis of bacterial cytosine deaminase.

Acta crystallographica. Section D, Biological crystallography·2001

Same author

Homing endonucleases: structural and functional insight into the catalysts of intron/intein mobility.

Nucleic acids research·2001

Same author

Structure of a factor VIII C2 domain-immunoglobulin G4kappa Fab complex: identification of an inhibitory antibody epitope on the surface of factor VIII.

Blood·2001

Same author

Trapping reaction intermediates in macromolecular crystals for structural analyses.

Methods (San Diego, Calif.)·2001

Same author

The homing endonuclease I-CreI uses three metals, one of which is shared between the two active sites.

Nature structural biology·2001

Same journal

Tomogram exploration through template matching and deep learning.

Current opinion in structural biology·2026

Same journal

A comparative review of cryo-electron ptychography: Biological applications and future perspectives.

Current opinion in structural biology·2026

Same journal

Metabolic disruptions through a three-dimensional genomic lens.

Current opinion in structural biology·2026

Same journal

Collective variable design for biomolecular conformational dynamics.

Current opinion in structural biology·2026

Same journal

Polymer scaling in protein crowding: From dilute coils to semidilute meshes.

Current opinion in structural biology·2026

Same journal

Tuning the physicochemical properties of rationally designed protein-based biomolecular condensates.

Current opinion in structural biology·2026

See all related articles

Recent time-resolved crystallography studies highlight the necessity of comparative analyses. Understanding protein intermediate structures requires diverse experimental approaches and trapping protocols.

Area of Science:

Structural Biology
Biochemistry

Background:

Time-resolved crystallography is crucial for observing transient protein states.
Characterizing reaction intermediates provides insights into protein dynamics and function.

Purpose of the Study:

To review recent advancements in time-resolved crystallography.
To emphasize the importance of parallel comparative studies for characterizing reaction intermediates.

Main Methods:

Determination of time-resolved crystallographic structures.
Application of various trapping protocols.
Utilization of diverse data collection methods.
Conducting parallel comparative studies on the same protein.

Main Results:

Related Experiment Videos

Several time-resolved structures of protein intermediates were determined.
Comparative studies revealed the structural variations of intermediates under different experimental conditions.

Conclusions:

Parallel comparative studies are essential for a comprehensive understanding of protein reaction pathways.
The choice of trapping protocols and data collection methods significantly influences the observed intermediate structures.