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Recent advances in site-directed spin labeling of proteins
W L Hubbell1, A Gross, R Langen
1Jules Stein Eye Institute, University of California, Los Angeles 90095, USA. hubbellw@jsei.ucla.edu
Current Opinion in Structural Biology
|November 18, 1998
Abstract:
Site-directed spin labeling of proteins is experiencing a phase of rapid technical evolution, application and evaluation. New strategies have been introduced for determining membrane protein topography, electrostatic potentials, the orientation of proteins at membrane surfaces and inter-residue distances. New applications include studies of beta strands, structure mapping using spin-spin interactions, domain motions in soluble proteins and extensive structural analysis of a number of membrane and soluble proteins.