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Development of a transformation system for the multinuclear yeast Dipodascus (Endomyces) magnusii
L Adamíková1, P Griac, L Tomáska
1Department of Biochemistry, Faculty of Natural Sciences, Comenius University, Bratislava, Slovakia.
Yeast (Chichester, England)
|November 18, 1998
Abstract:
We have developed the first system for genetic transformation of the multinuclear yeast Dipodascus magnusii. The system is based on a dominant selectable marker and an autonomously replicating sequence. We have constructed a plasmid vector which contains a marker conferring resistance to zeocin and the segment of non-transcribed spacer of D. magnusii ribosomal DNA which supports the autonomous replication of plasmid DNA in yeast cells. Plasmid DNA has been transferred into D. magnusii cells by electroporation.