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Related Experiment Videos

Tissue factor on cells

L V Rao1, U R Pendurthi

  • 1Department of Biochemistry, The University of Texas Health Center at Tyler, 75710, USA. rao@uthct.edu

Blood Coagulation & Fibrinolysis : an International Journal in Haemostasis and Thrombosis
|November 18, 1998
PubMed
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Tissue factor (TF) initiates blood clotting. Its functional expression is regulated by gene expression, cell membrane changes, and plasma inhibitors, crucial for hemostasis and thrombosis.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Hematology

Background:

  • Tissue factor (TF) is a cell surface glycoprotein essential for initiating the coagulation cascade.
  • TF binds factor VII (FVII) or activated factor VII (FVIIa), forming complexes that trigger blood clotting in vivo.
  • TF expression is typically low in vascular cells but can be induced by inflammatory and cancer-related stimuli.

Purpose of the Study:

  • To summarize the regulatory mechanisms of TF functional expression on cell surfaces.
  • To highlight recent findings on TF regulation by the authors and collaborators.
  • To emphasize the interplay between TF, FVIIa, and inhibitors in controlling coagulation.

Main Methods:

  • Review of transcriptional regulation of TF gene expression.

Related Experiment Videos

  • Analysis of cell membrane alterations affecting TF function.
  • Investigation of plasma inhibitors, including TF pathway inhibitor and antithrombin III, in TF-FVIIa regulation.

    • Main Results:

    • TF functional expression is modulated by transcriptional control, cell membrane properties (phospholipid availability, TF localization), and plasma inhibitors.
    • Specific stimuli can induce TF expression, while others, like basic fibroblast growth factor, can suppress it in endothelium.
    • Heparan sulfate proteoglycans enhance the activity of TF pathway inhibitor and antithrombin III, further regulating TF-FVIIa activity.

    Conclusions:

    • TF functional expression is a complex process regulated at multiple levels to maintain hemostasis and prevent thrombosis.
    • Understanding these regulatory mechanisms is critical for developing therapeutic strategies targeting TF in various diseases.
    • The findings underscore the intricate balance of procoagulant and anticoagulant factors in controlling blood coagulation.