A simple method for the serodiagnosis of human hydatid disease based on a protein A/colloidal dye conjugate

We have developed a dot immunobinding assay (CM) for the serodiagnosis of the human hydatidosis. The assay employs Samaron blue colloidal dye particles conjugated to hydatid antigen (HA) or protein A, both of which serve as visualizing agents. The test is simple and can be completed in 1 h. It requires few reagents, a nitrocellulose membrane (NC) strip onto which the HA has been bound, and the colored conjugates. It is sensitive and specific for the detection of anti-HA antibodies and generally agrees closely with the data from the enzyme-linked immunosorbent assay (ELISA).