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Related Experiment Videos

Polymerase chain reaction protocols for rabies virus discrimination

S A Nadin-Davis1

  • 1Rabies Centre of Expertise, Animal Diseases Research Institute, Canadian Food Inspection Agency, Nepean, Ont. nadindaviss@em.agr.ca

Journal of Virological Methods
|November 20, 1998
PubMed
Summary

Rapid molecular methods using RT PCR enable genetic characterization of rabies virus (RV) strains. These techniques are valuable for epidemiological studies and control programs, aiding in identifying variants and discriminating between RV reservoirs.

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Area of Science:

  • Veterinary Virology
  • Molecular Epidemiology
  • Public Health Microbiology

Background:

  • Rabies virus (RV) strains exhibit distinct host species reservoirs and geographic distributions.
  • Genetic characterization of RV is crucial for understanding rabies epidemiology and implementing effective control strategies.
  • The development of molecular techniques has significantly advanced the ability to differentiate RV strains.

Purpose of the Study:

  • To evaluate the utility of RT PCR-based methodologies for the genetic characterization and typing of rabies viruses.
  • To assess the application of molecular methods for rabies epidemiological studies and control programs in Ontario.
  • To identify intra-strain variants and discriminate between different RV reservoirs.

Main Methods:

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  • Reverse transcription polymerase chain reaction (RT PCR) for genetic analysis of rabies viruses.
  • Restriction fragment length polymorphism (RFLP) of PCR products for viral discrimination.
  • Strain-specific PCR (SS-PCR) using strain-specific primers for differential amplification.

    • Main Results:

    • RT PCR methodologies facilitate rapid genetic characterization of rabies viruses.
    • RFLP and SS-PCR provide valuable tools for RV typing and epidemiological studies.
    • These methods enabled identification of arctic fox strain variants and discrimination from bat and raccoon strains.
    • The raccoon strain's spread highlights the need for surveillance and rapid diagnostic tools.

    Conclusions:

    • Molecular methods based on RT PCR are effective for rabies virus strain typing and epidemiological surveillance.
    • These techniques are adaptable for use in regions with multiple overlapping RV reservoirs.
    • The developed methods support rabies control programs by enabling rapid identification of viral strains and variants.