Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Cellular localization of PDGF mRNAs in developing human forebrain

M Maxwell1, T Galanopoulos, J Neville-Golden

  • 1Neurosurgical Service, Massachusetts General Hospital and Harvard Medical School, Boston 02114, USA.

Neuropathology and Applied Neurobiology
|November 20, 1998
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Raising awareness of pre-conception care in community pharmacies: a feasibility study.

Pilot and feasibility studies·2022
Same author

Childhood adversity, mental health and suicide (CHASE): a methods protocol for a longitudinal case-control linked data study.

International journal of population data science·2021
Same author

Predicting Delayed Complications After Esophagectomy in the Current Era of Early Discharge and Enhanced Recovery.

The American surgeon·2020
Same author

Socioeconomic inequalities and the equity impact of population-level interventions for adolescent health: an overview of systematic reviews.

Public health·2020
Same author

Enhancing the get healthy information and coaching service for Aboriginal adults: evaluation of the process and impact of the program.

International journal for equity in health·2017
Same author

Protocol-developing meta-ethnography reporting guidelines (eMERGe).

BMC medical research methodology·2015
Same journal

Diagnostic Alteration Is Not Therapeutic Dependency: Lessons From Failed RET Inhibition in a PLAG-Altered CNS Embryonal Tumour.

Neuropathology and applied neurobiology·2026
Same journal

Respone to Škudar.

Neuropathology and applied neurobiology·2026
Same journal

Unravelling the Significance of Cystatin C and Bunina Bodies in Amyotrophic Lateral Sclerosis Pathogenesis.

Neuropathology and applied neurobiology·2026
Same journal

Neuropathological and Molecular Features Associated With a Heterozygous DNAJC7 Mutation in Amyotrophic Lateral Sclerosis.

Neuropathology and applied neurobiology·2026
Same journal

Central Nervous System Embryonal Tumour With PLAGL Amplification Lacking Typical Embryonal Morphology: Diagnostic Pitfall in a Paediatric Case.

Neuropathology and applied neurobiology·2026
Same journal

Lewy Bodies Are Not Associated With Neuronal or Synaptic Loss in Dementia With Lewy Bodies.

Neuropathology and applied neurobiology·2026
See all related articles

Platelet-derived growth factor (PDGF) plays a key role in human brain development. This study shows PDGF and its receptors are primarily expressed in embryonic glial cells, suggesting their importance in glial and neuronal development.

Area of Science:

  • Neuroscience
  • Developmental Biology
  • Cell Biology

Background:

  • Platelet-derived growth factor (PDGF) is involved in central nervous system (CNS) gliogenesis.
  • Previous rodent studies provided conflicting data on whether glia or neurons are the primary source of PDGF.

Purpose of the Study:

  • To investigate the in vivo expression and cellular localization of PDGF-A, PDGF-B, and their receptors (alpha and beta) in the developing human forebrain.

Main Methods:

  • In situ hybridization and immunocytochemical analysis were employed.
  • Counterstaining with glial cell (GFAP) and neuronal (NF) specific antibodies identified cell types.

Main Results:

  • Strong expression of PDGF-A, PDGF-B mRNA, and protein was observed in human embryonic glial cells.

Related Experiment Videos

  • Neurons exhibited lower levels of PDGF and PDGF-receptor mRNA and protein compared to glial cells.
  • Both PDGF chains and their receptors were predominantly expressed in glial cells.

    • Conclusions:

    • The predominant glial-specific expression of PDGF-A, PDGF-B, and their receptors suggests a significant role for these isoforms in the development of human embryonic glial cells and neurons.
    • This finding clarifies the cellular source of PDGF in human brain development.