[Mechanisms of hyperpolarization of plasma membrane of macrophages and astrocytes during activation]
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Updated: Oct 27, 2026

Inducing Plasticity of Astrocytic Receptors by Manipulation of Neuronal Firing Rates
Published on: March 20, 2014
I A Gamaleĭ1, K M Kirpichnikova, A M Ishchenko
1Institute of Cytology, Russian Academy of Sciences, St. Petersburg.
Abstract:
The hyperpolarization response of macrophages and glial cells (astrocytes, U118 cell line) to the action of inducers of reactive oxygen species (ROS) has been studied. Macrophages were stimulated with chemotactic peptide fMLP and platelet activation factor (PAF). Astrocytes were affected by complement component--anaphylatoxin C5a. The hyperpolarization response of both cell types depends on intracellular Ca2+ concentration and extracellular K+ concentration. Depletion of K+ concentration in the medium (1 mM KCl) or chelation of intracellular Ca2+ with Quin-2 (50 microM) significantly decreased the hyperpolarization response or caused depolarization of plasma membrane. Moreover, inhibition of Ca(2+)-dependent K(+)-channels with quinidine (50 microM) induced only a prolonged depolarization of both cell types. The data obtained permit a suggestion that macrophage and astrocyte hyperpolarization response to stimulation with ROS inducers involves O2.- mediated activation of Ca(2+)-dependent K(+)-channels.
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