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Updated: Aug 12, 2026

Transient Expression and Cellular Localization of Recombinant Proteins in Cultured Insect Cells
Published on: April 20, 2017
Engineering N-glycosylation pathways in the baculovirus-insect cell system
D L Jarvis1, Z S Kawar, J R Hollister
1Department of Molecular Biology, University of Wyoming, Laramie 82071-3944, USA. DLJarvis@uwyo.edu
Abstract:
The inability to produce eukaryotic glycoproteins with complex N-linked glycans is a major limitation of the baculovirus-insect cell expression system. Recent studies have demonstrated that metabolic engineering can be used to extend the glycoprotein processing capabilities of lepidopteran insect cells. This approach is being used to develop new baculovirus-insect cell expression systems that can produce more authentic recombinant glycoproteins and obtain new information on insect N-glycosylation pathways.
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