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A fluorescent double stain for visualization of neural tissue by confocal laser scanning microscopy
1Eberhard-Karls-Universität Tübingen, Anatomisches Institut, Abt. Zelluläre Neurobiologie, Germany. kroeger@anatu.uni-tuebingen.de
Journal of Neuroscience Methods
|November 20, 1998
Abstract:
We present a fast and simple method for a general, fluorescent double stain that differentially labels various cellular components and visualizes all cells in confocal laser scanning microscopy. The technique is useful for two- and three-dimensional visualization of neural tissue and facilitates quantification of a variety of neuroanatomical parameters. Examples from cerebellum and retina are shown to demonstrate the broad applicability.