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Beta-galactosidase in immobilized cells of gherkin Cucumis sativus L
1Garden of Medicinal Plants, Faculty of Pharmacy, Comenius University, Bratislava, Slovak Republic.
Acta Biochimica Polonica
|November 20, 1998
Abstract:
Cell suspensions of gherkin (Cucumis sativus L.) were permeabilized by Tween-80, and immobilized by glutaraldehyde. Beta-galactosidase showed pH optimum at 4.9 and temperature optimum at 58 degrees C. The enzyme catalysed hydrolysis was linear for 3 h with 60-68% conversion of the substrate. The cells characterized by high beta-galactosidase activity and stability on long-term storage showed valuable technological properties.