Binding of endonuclease VII to cruciform DNA. Visualization in the electron microscope
1Institute for Genetics of the University of Cologne, Zülpicher Strasse 47, D-50674 Köln, Germany.
The Journal of Biological Chemistry
|November 21, 1998
Abstract:
The binding of Holliday structure resolving endonuclease VII to cruciform DNA was studied in the electron microscope. The protein was found to bind either to the junction or to one of the arms or an end of one of the arms of the construct. The amount of bound protein was determined by measuring the size of the complexes. On average, one complex containing three dimers was found per one molecule of cruciform DNA.
