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Related Experiment Videos

Laser-micropipet combination for single-cell analysis

C E Sims1, G D Meredith, T B Krasieva

  • 1Department of Physiology and Biophysics, University of California, Irvine 92697, USA.

Analytical Chemistry
|November 21, 1998
PubMed
Summary
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A new laser technique rapidly lyses single cells for faster biochemical measurements. This method avoids cell stress, improving accuracy for analyzing rapidly changing intracellular molecules.

Area of Science:

  • Biochemistry
  • Cell Biology
  • Analytical Chemistry

Background:

  • Capillary electrophoresis (CE) offers high resolution for single-cell biochemical analysis.
  • Current CE methods face limitations in sampling speed for dynamic physiological measurements.
  • Mechanical or electrical stresses can alter cell physiology, compromising measurement accuracy.

Purpose of the Study:

  • To develop a faster cell lysis technique for single-cell capillary electrophoresis.
  • To enable accurate measurement of rapidly changing intracellular molecules.
  • To refine single-cell analysis by minimizing pre-sampling cellular perturbation.

Main Methods:

  • A laser-based technique was developed for millisecond-timescale lysis of single, adherent mammalian cells.

Related Experiment Videos

  • Cellular contents were introduced into a capillary for electrophoretic separation and detection.
  • A laser-micropipet combination was employed for precise cell manipulation and lysis.

    • Main Results:

    • The laser technique achieved rapid cell lysis on millisecond timescales.
    • This method introduced cellular contents into CE without prior mechanical or electrical stress.
    • The approach is applicable to adherent mammalian cells, enhancing temporal resolution.

    Conclusions:

    • The developed laser-based lysis technique significantly improves the speed of single-cell CE measurements.
    • Minimizing pre-sampling cellular stress leads to more accurate physiological parameter measurements.
    • This advancement allows for the study of intracellular molecules with subsecond to second dynamics.