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Related Experiment Videos

BRCA2 associates with acetyltransferase activity when bound to P/CAF

F Fuks1, J Milner, T Kouzarides

  • 1Wellcome/CRC Institute and Department of Pathology, Cambridge University, UK.

Oncogene
|November 21, 1998
PubMed
Summary

The BRCA2 gene, linked to hereditary breast cancer, interacts with the P/CAF co-activator. This interaction suggests BRCA2 regulates transcription by recruiting histone acetyltransferase activity.

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Area of Science:

  • Molecular Biology
  • Genetics
  • Cancer Research

Background:

  • Hereditary breast cancer is often linked to mutations in the BRCA2 gene.
  • The functional roles of the large BRCA2 protein remain incompletely understood.
  • Previous research indicated BRCA2 possesses transcriptional activation potential.

Purpose of the Study:

  • To investigate the functional interactions of the BRCA2 protein.
  • To explore the mechanism underlying BRCA2's transcriptional activation function.
  • To determine if BRCA2 interacts with histone acetyltransferase (HAT) proteins.

Main Methods:

  • In vitro and in vivo binding assays to detect BRCA2-P/CAF interaction.
  • Identification of the specific BRCA2 residues involved in P/CAF binding (290-453).

Related Experiment Videos

  • Assays to detect BRCA2 association with HAT activity in nuclear extracts.

    • Main Results:

    • BRCA2 directly interacts with the transcriptional co-activator P/CAF, a protein with histone acetyltransferase activity.
    • This interaction is mediated by specific residues within the BRCA2 protein (amino acids 290-453).
    • BRCA2 associates with acetyltransferase activity, supporting its role in transcription regulation.

    Conclusions:

    • BRCA2's transcriptional activation function is further supported by its interaction with P/CAF.
    • BRCA2 may regulate transcription by recruiting the histone-modifying activity of P/CAF.
    • This study elucidates a novel mechanism for BRCA2 in gene transcription, relevant to hereditary breast cancer.