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Changes in neutrophil surface phenotype during hemodialysis
K M Skubitz1, J Butterfield, K Ma
1Department of Medicine, University of Minnesota Medical School, Minneapolis, USA.
Inflammation
|November 24, 1998
Summary
Hemodialysis causes a temporary drop in neutrophils due to lung sequestration. Neutrophils desensitize to complement C5a stimulation in vivo during dialysis, explaining the transient neutropenia.
Area of Science:
- Immunology
- Nephrology
- Cell Biology
Background:
- Hemodialysis with cuprophane membranes induces rapid neutropenia.
- This neutropenia results from neutrophil sequestration in the lungs via homotypic aggregation, triggered by C5a generation.
- The transient nature suggests neutrophil desensitization in vivo.
Purpose of the Study:
- To investigate the in vivo effects of continuous C5a exposure on neutrophil surface phenotype during hemodialysis.
- To analyze changes in the expression of 22 surface antigens over a 3-hour dialysis period.
Main Methods:
- Flow cytometry was used to examine neutrophil surface antigen expression in patients undergoing hemodialysis.
- Blood samples were collected at 15, 60, and 180 minutes during dialysis.
Main Results:
- Neutropenia was observed at 15 minutes but resolved by 60 and 180 minutes.
- Several neutrophil surface antigens (e.g., CD10, CD11b, CD45) were upregulated early and remained elevated.
- Other antigens showed transient changes or no significant alteration during the 3-hour dialysis period.
Conclusions:
- Neutrophils undergo significant surface phenotype changes during prolonged in vivo exposure to C5a.
- Neutrophils become desensitized to C5a stimulation but retain responsiveness to other chemotactic stimuli.
- This desensitization mechanism contributes to the resolution of hemodialysis-induced neutropenia.
