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[Optimizing plasma cell content in bone marrow aspirates]

T Hoffmann1, G Favre, A Gratwohl

  • 1Departement Zentrallabor, Kantonsspital, Basel. thoffmann@uhbs.ch

Schweizerische Medizinische Wochenschrift
|November 24, 1998
PubMed
Summary
This summary is machine-generated.

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Secondary bone marrow aspirates, often used for flow cytometry, systematically underestimate plasma cell content due to increased blood contamination. This impacts accurate myeloma diagnosis and monitoring.

Area of Science:

  • Hematology
  • Immunology
  • Clinical Pathology

Context:

  • Flow cytometry is crucial for analyzing plasma cells in bone marrow.
  • Bone marrow samples are typically collected via aspiration.
  • Primary aspirates are often used for cytology, with secondary aspirates used for flow cytometry.

Purpose:

  • To investigate if secondary bone marrow aspirates lead to underestimation of plasma cell content.
  • To determine if increased blood contamination in secondary aspirates affects plasma cell quantification.
  • To compare plasma cell percentages between primary and secondary bone marrow aspirates.

Summary:

  • Plasma cell (CD38bright) percentages were analyzed in 13 pairs of primary and secondary bone marrow aspirates using flow cytometry.

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  • Secondary aspirates consistently showed lower plasma cell content compared to primary aspirates (p = 0.0015).
  • Median plasma cell counts were 57% lower in secondary aspirates, indicating systematic underestimation.

    • Impact:

    • The findings suggest that using secondary aspirates for flow cytometry can lead to a significant underestimation of bone marrow plasma cell burden.
    • This underestimation may have implications for the diagnosis and monitoring of plasma cell disorders like multiple myeloma.
    • Clinical laboratories should consider the source of bone marrow aspirates when interpreting flow cytometry results for plasma cell analysis.